How To Use Secure Seals TM
How to Use Secure SealsTM droplet of oil or nail polish over each port or by placing an HybriSlipTM over REMOVAL: 1. Grasp the edge of chamber firmly and peel the Secure Seal away from the microscope slide. SEALING: 2. Apply finger pressure to both tabs simultaneously for approximately five ... Retrieve Doc
Exercise #1: - Oglethorpe University
Microscope slides. Cover slips. Nail polish. Stock bottle of Drosophila virilis with third instar larvae. A water bath set at 37°C. Small tubes for heat shocking larvae. To preserve the slide, seal the edges with nail polish ... Document Viewer
Indirect Immunofluorescence For Budding Yeast
Indirect Immunofluorescence for Budding Yeast ice until ready to apply to microscope slides. 10. slip, avoiding bubbles, and seal with nail polish. Store slides at -20°C. ALTERNATIVE PERMEABILIZATION After aspirating the PBS-BSA, ... Get Document
Exercise #1: - Oglethorpe University
Microscope slides. Cover slips. Nail polish. Stock bottle of Drosophila virilis with third instar larvae. A water bath set at 37°C. Small tubes for heat shocking larvae. To preserve the slide, seal the edges with nail polish. EXAMPLES OF GOOD SQUASHES: ... Doc Retrieval
Chapter 14
Nail polish (to seal coverslips on slides). 1. Sterile, 1.5 or 2 mL centrifuge tubes. 2. PBT Mount guts on microscope slides in mounting solution or PBS/glycerol. 3.5 Gut Dissection 3.6 Live Imaging Philip L. Houtz and Nicolas Buchon. 179 ... Access Document
DROSOPHILA SALIVARY GLAND CHROMOSOMES
DROSOPHILA SALIVARY GLAND CHROMOSOMES page 41a David B. Fankhauser, PhD 16Jan92, rvsn 16Jan96, 6Jan97, microscope slides cover slips microscope immersion oil clear nail polish 1. ... Read Here
Lab 11: Transpiration - College Board
• Safety goggles, calculator, microscope, microscope slides, clear cellophane tape, clear nail polish, scissors • Graph paper and metric ruler as needed to determine leaf surface area investigation 11 tRansPiRation* What factors, ... Return Doc
Interphase FISH Protocol - Genetics
16. Seal each slide using nail polish 17. Allow at least 30’ for the nail polish to dry before imaging the slides to ensure no nail polish gets on the microscope objectives ... View Document
Chapter 4
Clear nail polish. 10 Seal the edges of the coverslips onto the microscope slides using clear nail polish. 12. The slide should be viewed under phase contrast to locate the cells and under confocal microscopy to image them. Alterna- ... Read More
Cover Slip - Wikipedia, The Free Encyclopedia
The cover slip can be glued to the slide so as to seal off the specimen, The cover slip is often glued to the microscope slide, A number of sealants are in use, including commercial sealants, laboratory preparations, or even regular clear nail polish, ... Read Article
Specimen Quality Slides/fixed Specimens
Drop of oil without it spreading and mixing with nail polish sealant. you should seal the edge of coverslip with nail polish to prevent liquid spilling onto microscope. Do not get any nail polish on the centre of the coverslip, ... Fetch Content
Wikipedia:Reference Desk/Archives/Science/2009 March 6 ...
My purpose is much more prosaic. Usually it's to secure and seal glass coverslips on microscope slides. It makes a reasonably hard, waterproof seal. when I was in lab they used to make us put "not for human use" on the nail polish we used for sealing slides. ... Read Article
PART XII - ELECTRON MICROSCOPE AND LIGHT MICROSCOPE SEROLOGY ...
- Rack for microscope slides - Blow-dryer - Petri dish with wet filter paper Seal the edges of the coverslip with melted paraffin wax or nail polish. ... Fetch Full Source
Preparation And Immunolabeling Of Caenorhabditis Elegans ...
Preparation and Immunolabeling of Caenorhabditis elegans Microscope slides Nail polish (for antibody incubation procedure) put an 18- x 18-mm #1 coverslip over the worms, and seal with nail polish. TROUBLESHOOTING Problem: There is high background staining. ... Doc Viewer
SLIDE & CASSETTE PROCESSING Coverslips (Cover Glasses)
Microscope Slides Slides, economy, 75 x 25 x 1 mm, Bx/72 Slides, Erie, 75 x 25 x 1 mm, Seal edges of coverslip with clear nail polish. A long nozzle, textured sides, and slim profile make this chemically inert, high-density ... Access Full Source
CONFOCAL MICROSCOPY OF LIPID RAFT CLUSTERING Reagents
Alexa Fluor 555-conjugated cholera toxin B subunit (Invitrogen) Olympus FV-300 FluoView Confocal Microscope Workstation (McGuire) underneath the cover slip and seal the edges with clear nail polish. 12. ... Doc Viewer
Immunofluorescent Staining Of FFPE Tissue Sections With ...
Immunofluorescent Staining of FFPE Tissue Sections with Antibodies Prepared Using the eFluor Nail polish. Instruments Microscope Wash the slides with gentle agitation in 1X PBS, ... Content Retrieval
Immunofluorescent Staining Of Intracellular Antigens On ...
Nail polish . Instruments Microscope (equipped with appropriate medium (Cat. No. 00-4958) and a coverslip. Seal coverslip with clear nail polish, allow to dry for 20 minutes in the dark. 11. For best results, image slides immediately. Slides can be stored at 4°C protected from light ... Get Doc
ReadiUse™ microscope Mounting Solution - AAT Bioquest
Term storage, seal the coverslip to the slide with nail polish or a plastic sealant. Mounted slides should be ReadiUse™ microscope mounting solution Author: Zhenjun Diwu Keywords: ... Fetch Here
The Tools Of The Trade - Texas A&M University
Nail Polish16 b. Super Glue Gel17 c. Duco Cement18 d. Epoxy 11. 1.0.0 9. Oils and Grease Seal glass capillaries in dry box. 24. microscope. 26. Amazon Keyword : slides with cavities . Good for holding samples covered by solvent. 27. ... Read More
Please Cite As: CSH Protocols; 2007; Doi:10
By confocal microscopy after staining the nuclei with propidium iodide. Nail polish. Screen, fine mesh (optional; For flat samples, such as mature leaves, sepals, or petals, use conventional microscope slides. ii. ... Return Doc
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