Hypoxia Studies With Pimonidazole In Vivo - Bio- Protocol
Superfrost plus; white microscope slides (Thermo Fisher Scientific, catalog number: 12-550-15) (VWR International, catalog number: 48393-106) 21. Tween 20 (Sigma-Aldrich, catalog number: P9416-50ML) 22. 0.9% saline (see Recipes) 23. 30 mg/ml pimonidazole in 0.9% saline (see Recipes) ... Retrieve Content
Distinct Patterns Of Serum Immunoreactivity As Evidence For ...
Onto Superfrost-plus glass microscope slides (VWR Inter-national, Poole, UK). Immunohistochemical staining using patient-derived sera Rat CNS tissue Human-derived sera were used as pri-mary antisera and incubated with both sagittal and coro- ... Access Doc
Apelin Is Required For Non-Neovascular Remodeling In The Retina
Vascular Biology, Atherosclerosis, and Endothelium Biology Apelin Is Required for Non-Neovascular Remodeling in the Retina Jenny A.G. McKenzie,* Marcus Fruttiger,* ... Retrieve Full Source
Effects Of High Salt Intake On Brain AT Receptor Densities In ...
Effects of high salt intake on brain AT 1 receptor densities in Dahl rats. Am J Physiol Heart were cut on a cryostat, thaw mounted onto Superfrost Plus microscope slides (VWR; West Chester, PA), and dehydrated overnight in a desiccator at 4°C. ... Fetch Content
Frozen Sectioning Of 3D Structures For Laser-capture ...
Under an inverted microscope to confirm that acini are on the slides 7. Cut 8 µm sections, Wick sections onto Superfrost plus slides (VWR #48311-703) and move the slide immediately to the slide rack inside the cryomicrotome box ... Read Document
Supporting Information - PNAS
Supporting Information Girard et al. 10.1073/pnas.1113166109 coated “D-coverslips” (18CIR.-1.5 D; VWR) or COS-7 and HeLa cells plated on poly-L-lysine were washed in PBS SuperFrost microscope slides (Menzel-Glaser) by using Ultra-Mount fluorescence mounting medium ... Access Doc
Oncorhynchus Mykiss - American Journal Of Physiology
Using a cryostat (Leica CM 1850) and placed onto glass microscope slides (VWR superfrost plus). Slides were dried at room temperature Oncorhynchus mykiss: comparative physiology and molecular evolution. J Exp Biol 208: 1951–1961, 2005. 12. ... Read More
The DNA Fiber Technique Visualization Of DNA Replication In ...
Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique Rebekka A.V. Schwab1, Microscope slides SuperFrost VWR international 631-0910 Cover slips No1 Scientific Laboratory Supplies LTD MIC3234 ... Access Full Source
Involvement Of G Protein-Coupled Receptor Kinase (GRK) 3 And ...
Coverslips used to grow cells were from VWR Scientific (West Ches-ter, PA), and the Superfrost microscope slides used to mount the coverslips were purchased from Fisher Scientific (Pittsburgh, PA). NG108 and BN17 cells were obtained from Dr. Graeme Milligan ... Fetch This Document
Comparison Of Adenoviral And Adeno-Associated Viral Vectors ...
Comparison of Adenoviral and Adeno-Associated Viral stat and placed on VWR Superfrost plus microscope slides (Erie Scientific Company, Portsmouth, NH). Tissue sections were fixed in 1.25% glutaraldehyde in PBS for 10 min and rinsed 3 ... Read Document
Extracellular Signal-Regulated Kinase 1/2-Mediated ...
Coverslips used to grow cells were from VWR Scientific (West Ches-ter, PA), and the Superfrost microscope slides used to mount the cover slips were purchased from Fisher Scientific (Pittsburgh, PA). Cell Culture. The neuroblastoma/glioma hybrid NG108 cells ... Visit Document
Supporting Information - PNAS
Supporting Information Hobley et al. 10.1073/pnas.1306390110 SuperFrost Ultra Plus adhesion microscope slides (VWR). The thickness cover glass (22 × 22 mm) and sealed with nail varnish. The slides were stored at −20 °C before analysis. Samples were ... Doc Retrieval
Chondrocyte Death By Apoptosis Is Associated With Cartilage ...
Company Ltd, Huntingdon, England), placed on Superfrost Plus microscope slides (VWR International, UK) before being left to air dry overnight. Population 2 consisted solely of equine left MCP joints (n¼9) also obtained from the local abattoir. ... Read Content
Laser Microdissection Expression Profiling Of Marginal Edges ...
Superfrost Plus microscope slides (VWR). Liver parenchyma was used as a positive control whilst omission of the primary antibody served as a negative control. Slides were de-waxed with xylene followed by dehydration in graded alcohols. ... Doc Viewer
Microscope slide - Wikipedia, The Free Encyclopedia
A microscope slide is a thin flat piece of glass, typically 75 by 26 mm (3 by 1 inches) Microscope slides are usually made of optical quality glass, such as soda lime glass or borosilicate glass, but specialty plastics are also used. ... Read Article
Increases In Brain And Cardiac AT Receptor And ACE Densities ...
Increases in brain and cardiac AT1 receptor and ACE densities after myocardial infarct in rats Junhui Tan, Hao Wang, and Frans H. H. Leenen Hypertension Unit, onto Superfrost Plus microscope slides (VWR, West Chester, PA) and stored at 80°C. ... Get Doc
Neuropilin-2 Promotes Branching Morphogenesis In The Mouse ...
Superfrost Plus microscope slides (48312-501, VWR International) were steamed for 30 minutes in a citrate buffer (pH 6.0; Zymed/Invitrogen, Carlsbad, CA, USA) for antigen retrieval. Endogenous peroxidase was quenched using 3% hydrogen peroxide (Sigma-Aldrich, St Louis, MO, ... Return Doc
Biofilm Formation By The Fish Pathogen : Development And
Four microscope glass slides [VWR ® Superfrost® Plus (VWR, Radnor, PA)] were 93 suspended vertically into the broth with the aid of metal clamps attached to a glass rod. 94 Approximately 3 cm of the glass slide was im mersed into the brot h. ... Read Document
Ragweed-induced Expression Of GATA-3, IL-4, And IL-5 By ...
(10–20 m) were placed on Superfrost Plus microscope slides (VWR Scientific, West Chester, PA), acetylated using 0.25% acetic acid anhydride, and dehydrated in ethanol. Sections were hybridized using synthetic DNA oligonucleotides with ... Retrieve Doc
Identification Of Chromatin Remodeling Genes Arid4a And ...
Cells by sliding tissue between two superfrost microscope slides. RBCs were lysed by hypotonic buffer (NH 4 M, pH 7.2). Cells from spleen or bone marrow were gently filtered through 70- µ m cell strainers (BD Falcon, VWR, Batavia, IL) and were stained with directly fluorescein-5 ... Document Viewer
Whole Mount Staining Bench Protocol (january 2015)
Collect 20µm frozen sections of the PFA fixed tissue of interest on superfrost slides. 2. Incubate the slides for 3h in 100% methanol 3. Secure the chamber to the microscope slide with Kwik-sil epoxy (VWR). This epoxy is ... Fetch This Document
No comments:
Post a Comment